期刊
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
卷 9, 期 4, 页码 811-816出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jpclett.7b03123
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资金
- National Research Foundation (NRF), Prime Minister's Office, Singapore [NRF-NRFI2016-03]
- Singapore Ministry of Education Academic Research Fund Tier 3 [MOE2012-T3-1-001, MOE2016-T3-1-002]
- National Natural Science Foundation of China [21708009]
- Fundamental Research Fund for the Central Universities [2017KFYXJJ153]
Many small protein domains or nucleic acid structures undergo two-state unfolding-refolding transitions during mechanical stretching using single-molecule techniques. Here, by applying the Jarzynski equality (JE), we analytically express the folding energy of a molecule as a function of the experimentally measured transition points xi* obtained with two typical time-varying mechanical constraints: the force constraints F(t) and the position constraints R(t) of a Hookian spring attached to one end of the molecule. Compared to previous applications of JE based on the integration of accurately measured force extension curves of a tether that typically contains the molecule of interest and handles, our approach just needs to accurately measure a single data point. In the case of the F(t) process, the calculation is handle-independent. The broad applications of the theory are demonstrated by measuring the folding energies of a DNA hairpin, a DNA G-quadruplex, and the titin 127 domain based on transition forces using magnetic tweezers.
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