期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 154, 期 -, 页码 75-84出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2018.02.028
关键词
Fluorescent sensor; Neomycin; Molecularly imprinted polymers; Boronate affinity; Quantum dots; Biological samples
资金
- National Natural Science Foundation of China [21277054, 21611130030]
- Hubei Province Health AMP
- Family Planning Scientific Research Project [WJ2015MA026, WJ2015X8004]
- Scientific Research Project of Wuhan city [2016060101010068]
A new analytical method was developed to detect neomycin in complex biological samples using molecularly imprinted polymer to construct an optical sensor. Fluorescent neomycin-imprinted polymers (fMIPs) containing both imprinted cavity and boronate affinity site were synthesized on the surface of silica-modified quantum dots. The fMIPs exhibited high selectivity to neomycin by having two binding sites for the target analyte. Neomycin analogues (competing for imprinted cavity) and D-glucose (competing for the boronate affinity site) did not affect the selectivity of the fMIPs. When combined with a fluorescent microplate reader, the obtained fMIP sensor displayed a linear concentration-dependent fluorescence quenching in response to neomycin in the range of 2-1000 mu g/L with a limit of detection as 0.16 mu g/L. The fMIP sensor was able to detect trace neomycin in biological samples accurately after simple sample pre-treatment. The sensitivity of the fMIP sensor was higher than HPLC equipped with a fluorescence detector. The fMIP sensor containing the doubly selective binding sites provides a selective, sensitive, accurate, and high through-put approach for neomycin monitoring. (C) 2018 Elsevier B.V. All rights reserved.
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