期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 159, 期 -, 页码 166-172出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2018.06.025
关键词
Quantitative; qNMR; Aluminum; Phosphate; Adjuvant; Vaccine
资金
- Government of Ontario through a MITACS [IT08656]
- Government of Canada through a MITACS [IT08656]
- Sanofi Pasteur Ltd.
A novel qNMR method is described for the quantitative determination of total aluminum and phosphate in aluminum phosphate (AlPO4) adjuvanted vaccine samples using solution Al-27 and P-31 nuclear magnetic resonance (NMR) spectroscopy. External standard calibrations of AIPO4 solutions established excellent linearity in the range of 15-40 x 10(-3) M and additional studies determined the level of detection for both nuclei. A commercialized combination vaccine product (Quadracel (R)), along with several individual adsorbed antigen components used in the vaccine were employed as model systems for method development. The developed method is also capable of quantitating the free phosphate (i.e. the fraction not bound to AlPO4 particles) in adjuvanted vaccines. This study is the first demonstration of a solution NMR method that is suitable for measuring total aluminum, and free and total phosphate concentrations in vaccine formulations consisting of antigen(s) adsorbed to aluminum adjuvant, in a single analytical workflow. (C) 2018 The Authors. Published by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据