期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 152, 期 -, 页码 128-136出版社
ELSEVIER
DOI: 10.1016/j.jpba.2018.01.046
关键词
Antioxidant activity; Flavonoids; Central composite rotatable design; Pinostrobin; Phytochemical profile
资金
- Croatian Science Foundation [HRZZ-IP-11-2013-8547]
The scope of current study was to apply supercritical CO2 for extraction of black poplar buds (Populus nigra L.) and to optimize the extraction parameters (pressure and temperature) using response surface methodology aimed at maximum extraction yield, total phenolic content (TP), ferric reducing antioxidant power (FRAP) and individual amount of selected bioactive phenolics. The pressure and temperature influenced significantly the extract yield (0.38-10.28 g/100 g), TP content (21.62-31.09 mu g GAE/mg), FRAP capacity (0.30-0.48 mu mol Fe2+/mg), and the amounts of p-coumaric acid (0.33-1.52 mu g/mg), pinocembrin (20.99-47.24 mu g/mg) and galangin (4.04-10.25 mu g/mg). The extraction of pinostrobin (51.73-79.56 mu g/mg), pinobanksin (0.41-1.55 mu g/mg), and chrysin (0.97-2.03 mu g/mg) was significantly affected only by the operating pressure. Optimized extraction parameters (30 MPa and 60 degrees C) allow to obtain higher yield and extracts enriched with bioactive phenolics. (C) 2018 Elsevier B.V. All rights reserved.
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