期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 156, 期 -, 页码 349-357出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2018.05.003
关键词
Metabolomics methods; Effective compounds; Honey-fried products; Network pharmacology; Farfarae flos; UHPLC-Q-TOF/MS
资金
- National Natural Science Foundation of China [81374050, 81503213]
- Special Science and Technology Foundation Project [2014FY111100]
- Tianjin Research Program of Application Foundation and Advanced Technology [15JCYBJC29300]
- Special Program of Talents Development for Excellent Youth Scholars in Tianjin of China
- PCSIRT [IRT-14R41]
This study aimed to clarify the difference between the effective compounds of raw and processed Farfarae flos using a network pharmacology-integrated metabolomics strategy. First, metabolomics data were obtained by ultra high-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UHPLC-Q-TOF/MS). Then, metabolomics analysis was developed to screen for the influential compounds that were different between raw and processed Farfarae flos. Finally, a network pharmacology approach was applied to verify the activity of the screened compounds. As a result, 4 compounds (chlorogenic acid, caffeic acid, rutin and isoquercitrin) were successfully screened, identified, quantified and verified as the most influential effective compounds. They may synergistically inhibit the p38, JNK and ERK-mediated pathways, which would induce the inhibition of the expression of the IFA virus. The results revealed that the proposed network pharmacology-integrated metabolomics strategy was a powerful tool for discovering the effective compounds that were responsible for the difference between raw and processed Chinese herbs. (C) 2018 Elsevier B.V. All rights reserved.
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