期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 148, 期 -, 页码 355-360出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2017.10.018
关键词
Scrophularia buergeriana; Neuroprotective effect; Cell extraction_LC-MS; Principal component analysis; HT22 cells
资金
- National Research Foundation of Korea Grant - Korean Government [NRF-2014R1A1A1003605, NRF-2014R1A4A1007304]
In the cell extraction_LC-MS method, when cells are incubated with natural product extracts, bioactive compounds selectively bind to extracellular or intracellular targets. The extracts and major compounds (phenylpropanoids and iridoid glycosides) of Scrophularia buergeriana Miguel have been reported to show neuroprotective effects both in vitro and in vivo. In this study, the cell extraction_LC-MS strategy was applied to screen and identify potential neuroprotective compounds from S. buergeriana by using immortalized mouse hippocampal HT22 cells. The results showed that two known compounds from S. buergeriana selectively bound HT22 cells. Additionally, metabolomics analyses were performed using the Mass Profiler Professional and Limma differential expression package of R to identify significant differences between HT22 cells treated with S. buergeriana and untreated cells. The cell extraction approach more accurately reflects in vivo conditions compared with other methods and can be readily used for screening bioactive components from natural products. (C) 2017 Elsevier B.V. All rights reserved.
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