期刊
JOURNAL OF PEPTIDE SCIENCE
卷 24, 期 8-9, 页码 -出版社
WILEY
DOI: 10.1002/psc.3092
关键词
DNA; peptide; peptide-oligonucleotide conjugate; self-assembly; solid-phase peptide fragment condensation
资金
- Yonsei University Future-Leading Research Initiative
- Graduate school of Yonsei University research scholarship grants in 2018
- Agency for Defense Development
- National Research Foundation (NRF) of Korea [2017R1A2A2A05069773]
- National Research Foundation of Korea [2017R1A2A2A05069773] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Peptide-oligonucleotide conjugates (POCs) are interesting molecules as they covalently combine 2 of the most important biomacromolecules. Sometimes, the synthesis of POCs involves unexpected difficulties; however, POCs with self-assembling propensity are even harder to synthesize and purify. Here, we show that solid-phase peptide fragment condensation combined with thiol-maleimide or copper-catalyzed azide-alkyne cycloaddition click chemistries is useful for the syntheses of self-assembling POCs. We describe guidelines for the selection of reactive functional groups and their placement during the conjugation reaction and consider the cost-effectiveness of the reaction. Purification is another important challenge during the preparation of POCs. Our results show that polyacrylamide gel electrophoresis under denaturing conditions is most suitable to recover a high yield of self-assembling POCs. This report provides the first comprehensive study of the preparation of self-assembling POCs, which will lay a foundation for the development of elegant and sophisticated molecular assemblies.
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