期刊
BIORESEARCH OPEN ACCESS
卷 3, 期 2, 页码 53-59出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/biores.2014.0001
关键词
apoptosis; cell cycle arrest; cell proliferation; cyclopamine; primary gastric cancer; Sonic Hedgehog
资金
- National Basic Research Program of China [2012cb114603]
- Research Fund for the Doctoral Program of High Education of China [20130182110003]
- Fundamental Research Funds for the Central Universities [XDJK2013B020]
The Sonic Hedgehog (Shh) signaling pathway is commonly activated in gastrointestinal cancer. However, our understanding of the Shh pathway in gastric cancer remains limited. Here we examined the effects of cyclopamine, a specific inhibitor of the Shh signaling pathway, on cell growth and proliferation in gastric primary cancer cells GAM-016 and the MKN-45 cell line. The results showed that the Shh signaling molecules SHH, PTCH, SMO, GLI1, and GLI2 were intact and activated in both types of cells. Furthermore, we observed that cyclopamine inhibited gastric cancer cell proliferation through cell cycle arrest and apoptosis. An in vivo study using NOD/SCID mouse xenografts demonstrated that cyclopamine significantly prevented tumor growth and development. Our study indicated that Shh signaling pathway could promote gastric cancer cell proliferation and tumor development, and blocking this pathway may be a potential strategy in gastric cancer treatment.
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