期刊
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
卷 59, 期 -, 页码 84-92出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jnutbio.2018.06.005
关键词
Chlorogenic acid; Intestinal barrier; Inflammation; Antioxidant capacity; Weaned pig
资金
- Special Fund for Agro-Scientific Research in the Public Interest [201403047]
- Youth Innovation teams of Animal Feed Biotechnology of the Sichuan Province [2016TD0028]
Intestinal barrier plays key roles in maintaining intestinal homeostasis. Inflammation and oxidative damage can severely destroy the intestinal integrity of mammals. Chlorogenic acid (CGA) is a natural polyphenol present in human diet and plants, possessing potent antioxidant and anti-inflammatory activities. This study was conducted to investigate the protective effects of CGA and its molecular mechanisms on intestinal barrier function in a porcine model. Twenty-four weaned pigs were allotted to two groups and fed with a basal diet or a basal diet containing 1000 mg/kg CGA. The results showed that CGA decreased serum D-lactate and diamine oxidase levels, and enhanced the expression and localization of claudin-1 protein in apical intercellular region of small intestinal epithelium. Interestingly, CGA significantly decreased the mucosa histamine and tryptase contents, as well as the tryptase-positive mast cell counts. Moreover, the expression levels of critical inflammation molecules (interleukin-1 beta, interleukin-6, tumor necrosis factor-alpha, and nuclear factor-kappa B) were down-regulated by CGA in jejunal and ileal mucosa. However, the expression levels of inflammation repressors (suppressor of cytokine signaling 1 and toll-interacting protein) were up regulated by CGA. Importantly, CGA decreased the malondialdehyde content but elevated glutathione peroxidase and catalase content in duodenal and jejunal mucosa. The expression levels of critical molecules in antioxidant signaling (nuclear factor erythroid-derived 2-related factor 2 and heme oxygenase-1) were elevated by CGA in duodenal and jejunal mucosa. These results suggested that CGA could ameliorate intestinal barrier disruption in weaned pigs, which might be mediated by suppressing the TLR4/NF-kappa B signaling pathway and activating the Nrf2/HO-1 signaling pathway. (C) 2018 Elsevier Inc. All rights reserved.
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