Simultaneous and sensitive detection of dopamine and uric acid using a poly(L-methionine)/gold nanoparticle-modified glassy carbon electrode

A novel electrochemical sensor was fabricated by electrodeposition of gold nanoparticles on a poly(L-methionine) (PMT)-modified glassy carbon electrode (GCE) to form a nano-Au/PMT composite-modified GCE (nano-Au/PMT/GCE). Scanning electron microscopy and electrochemical techniques were used to characterize the composite electrode. The modified electrode exhibited considerable electrocatalytic activity towards the oxidation of dopamine (DA) and uric acid (UA) in phosphate buffer solution (pH = 7.00). Differential pulse voltammetry revealed that the electrocatalytic oxidation currents of DA and UA were linearly related to concentration over the range of 5.0 x 10(-8) to 10(-6) mol/L for DA and 7.0 x 10(-8) to 10(-6) mol/L for UA. The detection limits were 3.7 x 10(-8) mol/L for DA and 4.5 x 10(-8) mol/L for UA at a signal-to-noise ratio of 3. According to our experimental results, nano-Au/PMT/GCE can be used as a sensitive and selective sensor for simultaneous determination of DA and UA. (c) 2014, Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.