期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 431, 期 1, 页码 34-47出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2018.06.037
关键词
dCas9; CRISPRi/a; gene regulation; epigenome engineering; genetic screening
资金
- Stanford Institution Funds
- Li Ka Shing Foundation
- Kate Li Foundation
- Pew Foundation
- Alfred P. Sloan Fellowship
- National Institutes of Health [DP5 OD017887, R01 DA036858]
Programmable control of gene expression is essential to understanding gene function, engineering cellular behaviors, and developing therapeutics. Beyond the gene editing applications enabled by the nuclease CRISPR-Cas9 and CRISPR-Cas12a, the invention of the nuclease-dead Cas molecules (dCas9 and dCas12a) offers a platform for the precise control of genome function without gene editing. Diverse dCas tools have been developed, which constitute a comprehensive toolbox that allows for interrogation of gene function and modulation of the cellular behaviors. This review summarizes current applications of the dCas tools for transcription regulation, epigenetic engineering, genome imaging, genetic screens, and chromatin immunoprecipitation. We also highlight the advantages and existing challenges of the current dCas tools in genetic engineering and synthetic biology, and provide perspectives on future directions and applications. (C) 2018 The Authors. Published by Elsevier Ltd.
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