期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 431, 期 1, 页码 102-110出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2018.05.005
关键词
Hematopoietic stem cells; MMEJ; NHEJ; HDR; CRISPR/Cas9
The efficient site-specific DNA double-strand breaks (DSB) created by CRISPR/Cas9 has revolutionized genome engineering and has great potential for editing hematopoietic stem/progenitor cells (HSPCs). However, detailed understanding of the variables that influence choice of DNA-DSB repair (DDR) pathways by HSPC is required for therapeutic levels of editing in these clinically relevant cells. We developed a hematopoietic-reporter system that rapidly quantifies the three major DDR pathways utilized at the individual DSB created by CRISPR/Cas9-NHEJ, MMEJ, and HDR and show its applicability in evaluating the different DDR outcomes utilized by human hematopoietic cell lines and primary human HSPC. (C) 2018 Elsevier Ltd. All rights reserved.
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