期刊
JOURNAL OF INVESTIGATIVE DERMATOLOGY
卷 138, 期 2, 页码 316-324出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jid.2017.07.842
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类别
资金
- National Institutes of Health [CA-16042, AI-28697]
- JCCC
- UCLA AIDS Institute
- UCLA School of Medicine
- K01 grant [AR071479]
Studies of the human skinmicrobiome suggest that Propionibacterium acnes strains may contribute differently to skin health and disease. However, the immune phenotype and functions of T helper type 17 (Th17) cells induced by healthy (PH) versus acne (PA) skin-associated P. acnes strains are currently unknown. We stimulated peripheral blood mononuclear cells from healthy donors and observed that PA strains induce higher IL-17 levels than PH strains. We next generated PH and PA strain-specific Th17 clones and show that P. acnes strains induce Th17 cells of varied phenotype and function that are stable in the presence of IL-2 and IL-23. Although PH-and PA-specific clones expressed similar levels of LL-37 and DEFB4, only PH-specific clones secreted molecules sufficient to kill P. acnes. Furthermore, electron microscopic studies showed that supernatants derived from activated PH and not PA-specific clones exhibited robust bactericidal activity against P. acnes, and complete breaches in the bacterial cell envelope were observed. This antimicrobial activity was independent of IL-26, because both natural IL-26 released by Th17 clones and rhIL-26 lacked antimicrobial potency against P. acnes. Overall, our data suggest that P. acnes strains may differentially modulate the CD4(+) T-cell responses, leading to the generation of Th17 cells that may contribute to either homeostasis or acne pathogenesis.
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