期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 153, 期 -, 页码 186-194出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2018.03.003
关键词
Litopenaeus vannamei; Lipopolysaccharide (LPS); Intestine; Immune; Digestive
类别
资金
- earmarked fund for Guangdong Natural Science Foundation [2017A030313147, 2015A030313855]
- Guangdong Provincial Special Fund for Marine Fisheries Technology [A201701B09]
- Fund of Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, P. R. China [FREU2017-01]
- Guangdong Provincial Key Laboratory of Fishery Ecology and Environment [LFE-2016-12]
- Central Public-interest Scientific Institution Basal Research Fund, South China Sea Fisheries Research Institute, CAFS [2016TS07, 2017YB12]
- National Natural Science Foundation of China [31502183, 31702352]
- Shenzhen Science and Technology Planning Project [JCYJ20170412110605075]
The shrimp intestine barrier serves as the first line of the host defense against pathogen infection. Lipopolysaccharide (LPS) is the cell wall component of gram-negative bacteria, which known as endotoxin and induce the intestine inflammation. In this study, the acute toxicity effects of LPS injection on the morphology, digestive enzymes and immunological responses of intestine from Litopenaeus vannamei was investigated. HE stain showed that LPS injection damaged the intestine connective and epithelium tissue. Specifically, a decrease in the activities of digestive enzymes including of amylase, lipase, trypsin and pepsin was observed. Moreover, LPS injection increased the content of oxidative stress parameters (center dot O-2(-) generation capacity, LPO, MDA and PC), and the expression of HIF-1 alpha gene. Alternatively, the antibacterial activities (PO and T-NOS), and the expression of the antibacterial genes (proPO, ALF, Toll and Imd) and pathogen pattern recognition genes (LGBP and Lec) increased at first and then decreased. Significant variations in anitioxidant enzyme activity of T-AOC, and the expression of the anitioxidant-related genes (SOD, HSP70 and Trx). These results revealed that LPS injection harmed the intestine barrier of L. vannamei by damaging intestine mucosal structure, increasing oxidative stress, and suppressing the digestive and immune status.
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