期刊
JOURNAL OF INFECTIOUS DISEASES
卷 218, 期 11, 页码 1792-1801出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiy416
关键词
gametocytes; naturally acquired immunity; Plasmodium falciparum
资金
- Centers of Biomedical Research Excellence Subcontract Award
- National Institute of General Medical Sciences [1P20GM104317-01]
- K24 Award from the National Institute of Allergy and Infectious Diseases, National Institute of Health [K24AI112964]
- Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institute of Health
Background. Antigametocyte-specific immune responses may regulate Plasmodium falciparum gametocyte density, providing the rationale for pursuing transmission-blocking vaccines (TBVs) that target gametocytes in the human host. Methods. To identify novel antigametocyte TBV antigens, we interrogated the gametocyte proteome with our whole proteome differential screening method using plasma from a treatment-reinfection study conducted in western Kenya. At the start of the high-transmission season, 144 males (12-35 years) were enrolled and treated with quinine and doxycycline, peripheral venous blood samples were obtained, volunteers were observed, and weekly blood films were obtained for 18 weeks to quantify gametocytemia. Using plasma pooled from individuals with low versus high gametocyte carriage, we differentially screened a P falciparum gametocyte stage complementary deoxyribonucleic acid expression library. Results. We identified 8 parasite genes uniquely recognized by gametocyte-resistant but not by gametocyte-susceptible individuals. Antibodies to one of these antigens, PfsEGXP, predicted lower gametocytemia measured over the 18-week transmission season (P=.021). When analyzed dichotomously, anti-PfsEGXP responders had 31% lower gametocyte density over 18 weeks of follow-up, compared with nonresponders (P=.04). Conclusions. PfsEGXP is one of the first reported gametocyte-specific target of antibodies that predict decreased gametocyte density in humans and supports our novel TBV antigen discovery platform.
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