期刊
JOURNAL OF INFECTION AND CHEMOTHERAPY
卷 24, 期 5, 页码 325-329出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jiac.2018.01.018
关键词
Extracellular activity; Intracellular activity; Legionella species; Minimum extracellular concentration inhibiting intracellular multiplication (MIEC); Minimum inhibitory concentration (MIC)
资金
- Shionogi Co., Ltd.
- Chugai Pharmaceutical Co., Ltd.
- Boehringer Ingelheim Japan, Inc.
- Daiichi Sankyo Co., Ltd.
- Ono Pharmaceutical Co., Ltd.
- Astellas Pharma Inc.
- Otsuka Pharmaceutical Co., Ltd.
- Pfizer Japan Inc.
- Taiho Pharmaceutical Co., Ltd.
- Taisho Toyama Pharmaceutical Co., Ltd.
- Japan Blood Products Organization
- Asahi Kasei Pharma Corporation
- Sumitomo Dainippon Pharma Co., Ltd.
- Meiji Seika Pharma Co., Ltd.
- Toyama Pharmaceutical Co., Ltd.
- Toyama Chemical Co., Ltd.
- Kyorin Pharmaceutical Co., Ltd.
- Fujifilm Pharma Co., Ltd.
- Iyaku (Medicine and Drug) Journal Co., Ltd.
The activities of various antibiotics against 58 clinical isolates of Legionella species were evaluated using two methods, extracellular activity (minimum inhibitory concentration [MIC]) and intracellular activity. Susceptibility testing was performed using BSYE alpha agar. The minimum extracellular concentration inhibiting intracellular multiplication (MIEC) was determined using a human monocyte-derived cell line, THP-1. The most potent drugs in terms of MICs against clinical isolates were levofloxacin, garenoxacin, and rifampicin with MIC90 values of 0.015 mu g/ml. The activities of ciprofloxacin, pazufloxacin, moxifloxacin, clarithromycin, and azithromycin were slightly higher than those of levofloxacin, garenoxacin, and rifampicin with an MIC90 of 0.03-0.06 mu g/ml. Minocycline showed the highest activity, with an MIC90 of 1 mu g/ml. No resistance against the antibiotics tested was detected. No difference was detected in the MIC distributions of the antibiotics tested between L. pneumophila serogroup 1 and L. pneumophila non-serogroup 1. The MIECs of ciprofloxacin, pazufloxacin, levofloxacin, moxifloxacin, garenoxacin, clarithromycin, and azithromycin were almost the same as their MICs, with MIEC90 values of 0.015-0.06 mu g/ml, although the MIEC of minocycline was relatively lower and that of rifampicin was higher than their respective MICs. No difference was detected in the MIEC distributions of the antibiotics tested between L. pneumophila serogroup 1 and L. pneumophila non-serogroup 1. The ratios of MIEC: MIC for rifampicin (8) and pazufloxacin (2) were higher than those for levofloxacin (1), ciprofloxacin (1), moxifloxacin (1), garenoxacin (1), clarithromycin (1), and azithromycin (1). Our study showed that quinolones and macrolides had potent antimicrobial activity against both extracellular and intracellular Legionella species. The present data suggested the possible efficacy of these drugs in treatment of Legionella infections. (C) 2018 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
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