期刊
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
卷 65, 期 -, 页码 343-348出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jiec.2018.05.005
关键词
Green solvent; Protein stabilization; Room temperature storage; Natural deep eutectic solvent; Designer solvent
资金
- National Research Foundation of Korea (NRF) - Ministry of Science and ICT (MSIT) [NRF-2016R1A2B4015056, 2017M3A9F5032628, S-2018-0373-000, 2010-0027955]
The inherent vulnerability of protein biologics to high temperature is the origin of their limited use in clinical settings where proper storage and handling systems are not available. Herein, we demonstrate the first application of natural deep eutectic solvents (NADES) as a temperature-stable storage medium for a real therapeutic protein, human interferon-alpha 2 (IFN-alpha 2). A formulation of IFN-alpha 2 based on a NADES composed of choline chloride and fructose at a 1:1 molar ratio (CCl:Fru) had no influence on the activity of IFN-alpha 2. The NADES provided an appropriate environment for effective protection of IFN-alpha 2 from thermal denaturation at mid (37 degrees C) and high (70 degrees C) temperatures during long-term (90 days) and short-term (2 h) storage. Protein stability in the presence of CCl:Fru was demonstrated by orthogonal or complementary methods, including in vitro activity assay, circular dichroism spectroscopy and extrinsic fluorescence spectroscopy. In view of their biocompatibility, customizable properties, and the simplicity of synthesis and use, NADESs are suggested as a green and improved short-term and long-term storage medium that could facilitate the development of room-temperature biologics. (C) 2018 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
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