期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 455, 期 -, 页码 24-33出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2018.01.013
关键词
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资金
- Bill and Melinda Gates Foundation [OPP1032817, OPP1146996, OPP1114729, OPP1032144]
- National Institute of Allergy and Infectious disease Division of AIDS Center for HIV/AIDS Vaccine Immunology-Immunogen Discovery (CHAVI-ID) [1R01AI102691, 1P01AI120756, UM-1 AI100645]
- Bill and Melinda Gates Foundation [OPP1032817, OPP1032144, OPP1114729] Funding Source: Bill and Melinda Gates Foundation
The Fc Array is a multiplexed assay that assesses the Fc domain characteristics of antigen-specific antibodies with the potential to evaluate up to 500 antigen specificities simultaneously. Antigen-specific antibodies are captured on antigen-conjugated beads and their functional capacity is probed via an array of Fe-binding proteins including antibody subclassing reagents, Fey receptors, complement proteins, and lectins. Here we present the results of the optimization and formal qualification of the Fc Array, performed in compliance with Good Clinical Laboratory Practice (GCLP) guidelines. Assay conditions were optimized for performance and reproducibility, and the final version of the assay was then evaluated for specificity, accuracy, precision, limits of detection and quantitation, linearity, range and robustness.
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