期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 343, 期 -, 页码 59-67出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jhazmat.2017.09.009
关键词
Antibiotic; Ciprofloxacin biodegradation; Thermophilic bacterium; Thermus sp.; Fluoroquinolones
资金
- China-Japan Research Cooperative Program [2016YFE0118000]
- National Natural Science Foundation of China [41373092]
- IUE, CAS [IUEZD201402, IUEQN201501]
- Scientific and Technological Major Special Project of Tianjin City [16YFXTSF00420]
- Industry Leading Key Projects of Fujian Province [2015H0044]
Ciprofloxacin (CIP) is an antibiotic drug frequently detected in manure compost and is difficult to decompose at high temperatures, resulting in a potential threat to the environment. Microbial degradation is an effective and environmentally friendly method to degrade CIP. In this study, a thermophilic bacterium that can degrade CIP was isolated from sludge sampled from an antibiotics pharmaceutical factory. This strain is closely related to Thermos thermophilus based on 16S rRNA gene sequence analysis and is designated C419. The optimal temperature and pH values for CIP degradation are 70 degrees C and 6.5, respectively, and an appropriate sodium acetate concentration promotes CIP degradation. Seven major biodegradation metabolites were identified by an ultra-performance liquid chromatography tandem mass spectrometry analysis. In addition, strain C419 degraded other fluoroquinolones, including ofloxacin, norfloxacin and enrofloxacin. The supernatant from the C419 culture grown in fluoroquinolone-containing media showed attenuated antibacterial activity. These results indicate that strain C419 might be a new auxiliary bacterial resource for the biodegradation of fluoroquinolone residue in thermal environments. (C) 2017 Published by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据