期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 350, 期 -, 页码 136-143出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jhazmat.2018.02.019
关键词
Earthworms; Biochar; Extracellular enzymes; Bioactivation; Soil pollution
资金
- Ministerio de Economia y Competitividad, Spanish Government [CTM2014-53915-R]
The activation of blochar (carbonaceous material generated by pyrolysis of biomass) with extracellular enzymes generated from soil biological processes was studied via a 2-month microcosm using earthworms. The isolation of biochar particles (post-incubated biochar) from earthworm-treated soils allowed to confirm an enrichment of biochar with extracellular enzymes associated to biogeochemical (alkaline phosphatase, beta-glucosidase and arylsulfatase) and bioremediadon pathways (carboxylesterase). The hydrolytic activity of this biochar incubated with earthworms was up to 8 times higher compared with that of control biochar (incubated in earthworm-free soils). More interestingly, the enzyme carboxylesterase was stabilized onto biochar surface as demonstrated by its unchanged hydrolytic activity after multiple water rinses of the post-incubated biochar, and the remaining activity following heat stress. Moreover, this biochar-bound carboxylesterase activity was sensitive to inhibition by organophosphorus (23-100% of control activity) and methyl carbamate pesticides (37-57%), demonstrating that bioactivated biochar with carboxylesterases may inactivate these agrochemicals by providing stable ligands of enzymatic nature.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据