期刊
JOURNAL OF GENERAL VIROLOGY
卷 99, 期 4, 页码 501-511出版社
MICROBIOLOGY SOC
DOI: 10.1099/jgv.0.001037
关键词
human parainfluenza virus type 2; V protein; caspase-1; interleukin-1 beta
The multifunctional V protein of human parainfluenza virus type 2 (hPIV2) plays important roles in controlling viral genome replication, inhibiting the host interferon response and promoting virus growth. We screened a yeast two-hybrid library using V protein as bait to identify host factors that are important for other functions of V. One of several positive clones isolated from HeLa cell-derived cDNA library encodes caspase-1. We found that the C-terminal region of V interacts with the C-terminal region of caspase-1 in mammalian cells. Moreover, the V protein repressed caspase-1 activity and the formation of interleukin-1 beta (IL-1 beta) in a dose-dependent manner. IL-1 beta secretion induced by wild-type hPIV2 infection in human monocytic THP-1 cells was significantly lower than that induced by recombinant hPIV2 lacking V protein or having a mutant V. These data suggest that hPIV2 V protein inhibits caspase-1-mediated maturation of IL-1 beta via its interaction with caspase-1.
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