期刊
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
卷 57, 期 3, 页码 386-393出版社
INST TECNOLOGIA PARANA
DOI: 10.1590/S1516-89132014005000004
关键词
enzymatic hydrolysis; bioactive peptides; angiotensin I-converting enzyme inhibitors; peptide sequence
类别
资金
- Comite para el Desarrollo de la Investigacion (CODI) of Universidad de Antioquia [MDC 09-1-05]
The angiotensin I-converting enzyme (ACE) inhibiting activity of bovine plasma hydrolyzates obtained by Alcalase 2.4 L at different degrees of hydrolysis (DH) was evaluated. For the evaluation of ACE inhibition (ACEI), Hippuryl-His-Leu was used as substrate and the amount of hippuric acid liberated by non-inhibiting ACE was determined by spectrophotometry at 228 nm. The results showed that the enzymatic hydrolysis increased the ACEI activity as compared with the un-hydrolyzed plasma. The highest activity was onbtained with a DH of 6.7%. The peptide fractions with the maximum activity were isolated using ultrafiltration membranes, ion exchange chromatography and high performance liquid chromatography on reverse phase (RP-HPLC). The fraction with highest ACEI activity, showed an IC50 of 0.18 mg/mL and contained peptides with sequences AGATGVTISGAG, YSRRHPEYAVS, Q(K) AW and L(l) I(I) VR, which were determined by MALDI-TOF-TOF. It was also found that after submitting such fraction to digestive conditions in vitro, the ACEI activity remained constant.
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