期刊
JOURNAL OF FOOD SCIENCE
卷 83, 期 6, 页码 1516-1521出版社
WILEY
DOI: 10.1111/1750-3841.14175
关键词
antioxidant activity; bioactive peptides; cytoprotection; nsLTP2; triticum aestivum
资金
- BACCHUS project (FP7 European Commission Grant) [312090]
- Fondazione del Monte di Bologna e Ravenna (Italy)
- Biotechnology and Biological Sciences Research Council UK [BB/J004545/1]
- Montana Prize for Food Research
- Biotechnology and Biological Sciences Research Council [BBS/E/F/00042675, BBS/E/F/00044434, BBS/E/F/000PR10343] Funding Source: researchfish
- BBSRC [BBS/E/F/000PR10343, BBS/E/F/00044434, BBS/E/F/00042675] Funding Source: UKRI
Numerous studies support the protective role of bioactive peptides against cardiovascular diseases. Cereals represent the primary source of carbohydrates, but they also contain substantial amounts of proteins, therefore representing a potential dietary source of bioactive peptides with nutraceutical activities. The analysis of wheat extracts purified by chromatographic techniques by means of HPLC-UV/nanoLC-nanoESI-QTOF allowed the identification of a signal of about 7 kDa which, following data base searches, was ascribed to a nonspecific lipid-transfer protein (nsLTP) type 2 from Triticum aestivum (sequence coverage of 92%). For the first time nsLTP2 biological activities have been investigated. In particular, in experiments with human umbilical vein endothelial cells (HUVEC), nsLTP2 displayed antioxidant and cytoprotective activities, being able to significantly decrease reactive oxygen species (ROS) levels and to reduce lactate dehydrogenase (LDH) release, generated following oxidative (hydrogen peroxide) and inflammatory (tumor necrosis factor , interleukin-1, and lipopolysaccharide) stimulation. The obtained promising results suggest potential protective role of nsLTP2 in vascular diseases prevention. Practical ApplicationnsLTP 2 peptide is resistant to proteases throughout the gastrointestinal tract and exerts antioxidant and cytoprotective activities. These characteristics could be exploited in vascular diseases prevention.
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