Optimization of Extraction of Hypoglycemic Ingredients from Grape Seeds and Evaluation of α-Glucosidase and α-Amylase Inhibitory Effects In Vitro

Grape seeds full with oil and also rich in hypoglycemic ingredients. This study is to improve the comprehensive utilization of grape seeds and the quality of grape seeds oil. Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 +/- 1.1, 658.8 +/- 0.2, 482.3 +/- 0.2 (mg GSAE/g). Also we found GSAE samples containing catechin and epicatechin were 44.12 +/- 0.21 mg/mL, 111.23 +/- 1.29 mg/g, GSAE against alpha-glucosidase IC50 was 25.25 +/- 0.53 g/mL and GSAE against alpha-amylase IC50 was 66.68 +/- 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose. In addition, our process guarantee the fat ingredients remained in the grape seeds and not reduce functional components of oil, our study suggests that GSAE can be used to develop functional foods for prevention and treatment of diabetes and its complications.