Proximate composition, lipid utilization and validation of a non-lethal method to determine lipid content in migrating American shad Alosa sapidissima

Lipid content forms the most important energy reserve in anadromous fish and can limit survival, migration and reproductive success. A fat meter was evaluated and compared with a traditional extractive method of measuring available lipid for migrating American shad Alosa sapidissima in the Connecticut River, U.S.A. The fat meter gives rapid (<10s) and non-lethal lipid measurements, whereas traditional methods require lethal sampling that is both time consuming and expensive. The fat-meter readings had a strong relationship to traditional lipid extractions for 60 fish, 30 whole body (R-2=072) and 30 fillet only (R-2=081). Additional validation showed that fat-meter readings captured the gradual decrease of lipid in individual fish over time, were not affected by removal of gonads or scales and were stable for fish exposed to water or air for 24h after death. These experiments indicate that the fat meter can be used as a reliable tool for future A. sapidissima energetic studies, allowing for larger sample sizes and non-lethal sampling.