期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 69, 期 15, 页码 3573-3586出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/ery145
关键词
Auxin; carotenoid; cytokinin; fleshy fruit; phytochrome; plastid division; tomato; Solanum lycopersicum; starch
资金
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) [442045/2014-0]
- FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo) [2013/18056-2, 2016/01128-9]
Light signaling has long been reported to influence fruit biology, although the regulatory impact of fruit-localized photoreceptors on fruit development and metabolism remains unclear. Studies performed in phytochrome (PHY)deficient tomato (Solanum lycopersicum) mutants suggest that SlPHYA, SlPHYB2, and to a lesser extent SlPHYB1 influence fruit development and ripening. By employing fruit-specific RNAi-mediated silencing of SlPHY genes, we demonstrated that fruit-localized SlPHYA and SlPHYB2 play contrasting roles in regulating plastid biogenesis and maturation in tomato. Our data revealed that fruit-localized SlPHYA, rather than SlPHYB1 or SlPHYB2, positively influences tomato plastid differentiation and division machinery via changes in both light and cytokinin signaling-related gene expression. Fruit-localized SlPHYA and SlPHYB2 were also shown to modulate sugar metabolism in early developing fruits via overlapping, yet distinct, mechanisms involving the co-ordinated transcriptional regulation of genes related to sink strength and starch biosynthesis. Fruit-specific SlPHY silencing also drastically altered the transcriptional profile of genes encoding light-repressor proteins and carotenoid-biosynthesis regulators, leading to reduced carotenoid biosynthesis during fruit ripening. Together, our data reveal the existence of an intricate PHY-hormonal interplay during fruit development and ripening, and provide conclusive evidence on the regulation of tomato quality by fruit-localized phytochromes.
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