期刊
JOURNAL OF ETHNOPHARMACOLOGY
卷 222, 期 -, 页码 21-33出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2018.04.036
关键词
Apoptosis; Cameroon; Curcuma longa cytotoxicity; Lycopersicon esculentum, Psidium guajava
资金
- Alexander von Humboldt Foundation
Ethnopharmacological relevance: Recommendations have been made stating that ethnopharmacological usages such as immune and skin disorders, inflammatory, infectious, parasitic and viral diseases should be taken into account if selecting plants for anticancer screening, since these reflect disease states bearing relevance to cancer or cancer-like symptoms. Cameroonian medicinal plants investigated in this work are traditionally used to treat cancer or ailments with relevance to cancer or cancer-like symptoms. Aim of the study: In this study, 21 methanol extracts from 18 Cameroonian medicinal plants were tested in leukemia CCRF-CEM cells, and the best extracts were further tested on a panel of human cancer cell lines, including various multi-drug-resistant (MDR) phenotypes. Mechanistic studies were performed with the three best extracts. Materials and methods: Resazurin reduction assay was used to evaluate cytotoxicity and ferroptotic effects of methanol extracts from different plants. Flow cytometry was used to analyze cell cycle, apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) of extracts from Curcuma longa rhizomes (CLR), Lycopersicon esculentum leaves (LEL), and Psidium guajava bark (PGB). Results: In a pre-screening of all extracts, 13 out of 21 (61.9%) had IC50 values below 80 mu g/mL. Six of these active extracts displayed IC50 values below 30 mu g/mL: Cola pachycarpa leaves (CPL), Curcuma longa rhizomes (CLR), Lycopersicon esculenturn leaves, Persea americana bark (PAB), Physalis peruviana twigs (PPT) and Psidium guajava bark (PGB). The best extracts displayed IC50 values from 6.25 mu g/mL (against HCT116 p53(-/-)) to 10.29 mu g/mL (towards breast adenocarcinoma MDA-MB-231-BCRP cells) for CLR, from 9.64 mu g/mL (against breast adenocarcinoma MDA-MB-231 cells) to 57.74 mu g/mL (against HepG2 cells) for LEL and from 1.29 mu g/mL (towards CEM/ADR5000 cells) to 62.64 mu g/mL (towards MDA-MB-231 cells) for PGB. CLR and PGB induced apoptosis in CCRF-CEM cells via caspases activation, MMP depletion and increase ROS production whilst LEL induced apoptosis mediated by caspases activation and increase ROS production. Conclusion: The best botanicals tested were CLR and LEL, which are worth to be explored in more detail to fight cancers including MDR phenotypes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据