Surface-enhanced Raman spectroscopic analysis of N6-benzylaminopurine residue quantity in sprouts with gold nanoparticles

A rapid and quantitative method for the determination of N-6-Benzylademine (N-6-BA) was established through the application of surface-enhanced Raman spectroscopy (SERS). The Raman peak intensities of N-6-BA at 1002cm(-1) positively correlated to N-6-BA concentrations in sprout extracts. The R-2 reached 0.99, and RSDs calculated below 10% at the concentration range of 0.1 approximate to 5g mL(-1). The average recoveries were 80.0% approximate to 98.2% for blank samples intentionally contaminated at differing levels of 0.04, 0.4, and 1g g(-1). The whole procedure, including sample preparation and SERS detection, did not exceed 30min for a set of 6 samples. This study indicates that SERS is a promising technique for rapid tracing analysis and on-site testing of N-6-BA.