Deep exploitation of refractory organics in anaerobic dynamic membrane bioreactor for volatile fatty acids production from sludge fermentation: Performance and effect of protease catalysis

Volatile fatty acids (VFAs) production from waste activated sludge fermentation could be improved in anaerobic dynamic membrane bioreactor (ADMBR) by retaining residual organics within the reactor and prolonging their reaction time. However, the accumulation of refractory organics made it operate unstably. Therefore, protease catalysis was adopted to deeply exploit those refractory organics in sludge. By combining with dynamic membrane retention, protease catalysis indeed presented a good performance. VFAs yield was further enhanced by over 40% in ADMBR. Membrane fouling was slightly relieved due to protein and polysaccharide degradations in the sludge of dynamic membrane. It was also interestingly found that not only protease activity of sludge was improved from 5 to 21 U/ml, but also beta-GLC activity was enhanced from 13 to 20 mu moL/L/h. Microbial community analysis showed protease addition could reduce bacterial richness and evenness in sludge, and accelerate the growth of polysaccharides hydrolyzing bacteria, as well as inhibit some proteolytic bacteria. (C) 2018 Elsevier Ltd. All rights reserved.