期刊
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
卷 812, 期 -, 页码 1-9出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2017.12.012
关键词
Circulating tumor cells; Electrochemical cytosensor; MCF-7; Reduced graphene oxide/gold nanoparticles composites; CuO nanozyme
资金
- National Nature Science Foundation of China [81571812]
- Priority Academic Program Development of Jiangsu Higher Education Institutions [1107047002]
An ultrasensitive electrochemical detection method was developed to detect circulating tumor cells (CTCs) by using reduced graphene oxide/gold nanoparticles composites (rGO/AuNPs composites) as a support material with CuO nanozyme as a catalyst. MCF-7 circulating tumor cells were detected by an electrochemical cytosensor with effective surface recognition between specific mucin 1 protein (MUC-1) over-expressed on the MCF-7 cell membranes and MUC-1 aptamer. The CuO nanozyme is used as a signal-amplifying nanoprobe in an ultra sensitive electrochemical cytosensor to detect CTCs for the first time. Under the optimized experimental conditions, the proposed cytosensor exhibited significant analytical performance for the determination of MCF-7 circulating tumor cells. A wide detection range from 50 to 7 x 10(3) cells mL(-1) with a detection limit as low as 27 cells mL(-1) was reached on the condition of acceptable selectivity and reproducibility. Furthermore, the cytosensor can easily distinguish CTCs from the real serum sample due to the specific combination of MUC-1 and MUC-1 aptamer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据