期刊
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
卷 812, 期 -, 页码 180-185出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2017.11.059
关键词
Lipase; Electrodeposition; Nanoporous gold; Enzyme immobilisation; Flow cell; Microfluidic enzymatic reactor
资金
- European Union's Seventh Framework Programme for research, technological development and demonstration [607793]
- Government of Ireland Postgraduate Scholarship [GOIPG/2014/659]
- Irish Research Council (IRC) [GOIPG/2014/659] Funding Source: Irish Research Council (IRC)
An electrochemically triggered sol-gel process was used to generate a thin silica layer for the immobilisation of lipase from Thermomyces lanuginosus onto dealloyed nanoporous gold (NPG). The catalytic response of the entrapped lipase was examined using the hydrolysis of 4-nitrophenyl butyrate (4-NPB) as a model reaction. For the electrodeposition process, parameters including the deposition time and the concentration of lipase affected the observed catalytic activity. A deposition time of 180 s and a lipase concentration of 3 mg(-1) were used to prepare the optimised electrode. The operational stability of the silica immobilised enzyme was enhanced on NPG in comparison to that on planar gold, which may arise from confinement of the enzyme in the porous structure. The modified electrodes were incorporated into a 3D printed flow cell with conversion efficiencies of up to 100% after 8 cycles.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据