期刊
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
卷 44, 期 -, 页码 19-26出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jddst.2017.11.022
关键词
Mannosylated chitosan nanoparticles; rHBsAg; Cytotoxicity; Immune system
资金
- Tehran University of Medical Sciences and Health Services [94-01-87- 26598]
The design of effective vaccine delivery system is opening up new feasibilities for making immunization more safe and efficient. In this study, recombinant hepatitis B virus surface antigen (rHBsAg) was loaded in mannosylated chitosan (MC) nanoparticles to be used as a targeted vaccine delivery vehicles. The nanoparticles were prepared by ionic gelation method and characterized for physicochemical properties, cytotoxicity and antigenicity. The rHBsAg-loaded MC nanoparticles showed spherical shape with mean particle size of 246 +/- 33 nm, zeta potential of 25.6 +/- 1.7 mV, loading capacity of 12.2 +/- 1.4%, and encapsulation efficiency of 90 +/- 1.6%. In vitro release profile of rHBsAg-loaded MC nanoparticles exhibited an initial burst release of about 26% in the first 7 days followed by a slow release of 25% for 49 days, with release kinetic similar to Higuchi model. SDS-PAGE analysis confirmed integrity of released rHBsAg and structural stability of the antigen during entrapment process. The rHBsAg-loaded MC nanoparticles indicated time-and concentration-dependent cytotoxicity using MTT assay. It can be concluded that entrapment of rHBsAg in MC nanoparticles appears to be a suitable approach for targeting this antigen into body immune system.
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