期刊
JOURNAL OF CONTROLLED RELEASE
卷 282, 期 -, 页码 140-147出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2018.02.043
关键词
Nonviral gene delivery; Cationic polymers; T lymphocytes
资金
- National Institutes of Health [1R01CA177272, 2R01NS064404]
- National Science Foundation [DGE-1256082]
The clinical success of chimeric antigen receptor (CAR) T cell immunotherapy in treating multiple blood cancers has created a need for efficient methods of ex vivo gene delivery to primary human T cells for cell engineering. Here, we synthesize and evaluate a panel of cationic polymers for gene delivery to both cultured and primary human T cells. We show that a subset of comb-and sunflower-shaped pHEMA-g-pDMAEMA polymers can mediate transfection with efficiencies up to 50% in the Jurkat human T cell line with minimal concomitant toxicity (> 90% viability). We then optimize primary human T cell transfection conditions including activation time, cell density, DNA dose, culture media, and cytokine treatment. We demonstrate transfection of both CD4(+) and CD8(+) primary human T cells with messenger RNA and plasmid DNA at efficiencies up to 25 and 18%, respectively, with similarly high viability.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据