期刊
EUROPEAN JOURNAL OF MEDICAL RESEARCH
卷 19, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/2047-783X-19-41
关键词
Paris saponin VII (PS VII); Cervical cancer; Proliferation; Apoptosis
资金
- Technology Gallery of Shaanxi Province [2014JQ4152]
- Department of Public Health of Shaanxi Province [2012D45]
Background: Saponins of several herbs are known to induce apoptosis in many cancer cells. The present study aimed to investigate the growth inhibitory effect of Paris saponin VII (PS VII), a kind of steroidal saponins from Chonglou (Rhizoma Paridis Chonglou), on the human cervical cancer cell line Hela and the relative molecular mechanisms. Methods: Hela cells were exposed to different concentrations of PS VII (1 to 100 M). Inhibition of cell proliferation was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 5-ethynyl-2'-deoxyuridine (EdU) assays. The amount of apoptotic cells was evaluated by flow cytometric analysis. And the protein level of cleaved caspase-3, cleaved caspase-9, Bax, and Bcl-2 was evaluated by Western blot. Results: The half maximal inhibitory concentration (IC50) value of PS VII for the growth inhibition of Hela cells was 2.62 +/- 0.11 mu M. PS VII increased the expression of caspase-3, caspase-9, and Bax while decreased that of Bcl-2, suggesting that PS VII may induce apoptosis through intrinsic apoptotic ways. Conclusions: These data indicate that PS VII has the potential for the treatment of cervical cancer.
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