期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 56, 期 10, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00405-18
关键词
mumps virus; real-time RT-PCR; next-generation sequencing; molecular homology modelling; genotype; vaccines
类别
资金
- Canterbury Health Laboratories
Here, we report how the analysis of viral genetic variation using next-generation sequencing (NGS) can be used as a tool to improve mumps virus diagnostics. Analysis of NGS data from recently circulating mumps virus isolates allowed optimization of the current mumps virus real-time reverse transcription-PCR (RT-PCR) by primer and probe modifications due to nucleotide variations. The modified assay showed a higher efficiency and sensitivity than the previously used CDC protocol for the detection of currently circulating mumps virus strains and could therefore offer better support for outbreak control. The NGS sequence data were also used to make predictions of changes in the hemagglutinin-neuraminidase protein structure that could explain possible immune escape mechanisms.
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