期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 1092, 期 -, 页码 152-157出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2018.06.013
关键词
-
资金
- Irish Research Council [GOIPG/2015/3681]
- Irish Research Council (IRC) [GOIPG/2015/3681] Funding Source: Irish Research Council (IRC)
A dispersive liquid-liquid microextraction (DLLME) method, combined with HPLC-UV detection, was developed for the extraction and preconcentration of delta-tocopherol from bovine milk. This method was used to study the effect of supplementing cow feed with the seaweed Ascophyllum nodosum on vitamin content in milk. The optimal experimental conditions were determined: 200 mu L of chloroform (extraction solvent), 1.0 mL of ethanol (dispersive solvent), 5 mL of water (aqueous phase). Under these optimal conditions the DLLME method provided linearity in the range 0.01 mu g/mL to 8 mu g/mL with R-2 values of 0.998. Limit of detection (LOD) was 0.01 mu g/mL, while the enrichment factor was 89. Cow feed that was supplemented with Ascophyllum nodosum was shown to increase delta-tocopherol levels from 3.82 mu g/mL to 5.96 mu g/mL.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据