期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 1086, 期 -, 页码 153-165出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2018.04.013
关键词
Antibiotic drugs; Reversed phase high performance liquid chromatography; Mass spectrometry; Metabolites; Solid phase microextraction
资金
- National Science Centre (Cracov, Poland) [2016/21/B/ST4/02130]
A sensitive, rapid and specific analytical method using high performance liquid chromatography coupled with mass spectrometry (HPLC-QqQ-MS) was developed to determine selected antibiotic drugs and their metabolites (amoxicillin, cefotaxime, ciprofloxacin, dindamycin and metronidazole; amoxycilloic acid, 4-hydroxyphenyl glycyl amoxicillin, desacetyl cefotaxime, 3-desacetyl cefotaxime lactone, ciprofloxacin N-oxide, N-demethyl-clindamycin, clindamycin sulfoxide, and hydroxy metronidazole) in human whole blood and vascularized tissue after single oral administration. The samples were prepared by solid phase microextraction with C18 fibers (SPMEc18) and determined on a GRACE analytical C18 column, Vision HT (50 x 2 mm, 1.5 mu m) at the flow rate of 0.4 mL min(-1) using water and acetonitrile (containing 0.1% formic acid) as the mobile phase. The proposed method was successfully applied in a pharmacokinetic study of the selected antibiotic drugs and their metabolites in real human samples. Additionally, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS) was used for identification and qualification analysis of the target compounds.
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