Analysis of 10 beta-agonists in pork meat using automated dispersive pipette extraction and LC-MS/MS

An analytical procedure for the analysis of 10 beta-adrenergic agonists (cimaterol, terbutaline, salbutamol, isoxsuprine, ractopamine, cimbuterol, clenbuterol, brombuterol, mabuterol and mapenterol) in pork meat was developed and validated using LC-MS/MS. An automated dispersive pipette extraction (DPX) was employed on a Hamilton Microlab(center dot) NIMBUS96(center dot) platform to extract the analyses of interest prior to LC-MS/MS analysis. The extraction time was < 20 min with a total LC-MS/MS run time of 9.6 min. The method was fully validated in accordance with the international guidelines (European Commission Decision 2002/657/EC and National Standards of Peoples Republic of China, GB/T 22286-2008) for limit of detection, limit of quantitatlon, carryover, extraction efficiency, matrix effects, linearity, and within and between-run precision. The proposed method can be successfully used in the routine determination of 10 beta-adrenergic agonists in pork and as a potential solution for compliance monitoring in regulatory laboratories.