期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1545, 期 -, 页码 22-31出版社
ELSEVIER
DOI: 10.1016/j.chroma.2018.02.055
关键词
Cocaine; Ion mobility spectrometry; Magnetic nanoparticles; Magnetic molecularly imprinted polymer; Saliva
资金
- Ministerio de Economia y Competitividad [CTQ 2014-52841-P, CTQ 2014-52765-R]
- Generalitat Valenciana [PROMETEO-II 2014-077, PROMETEO/2016/145]
Magnetic molecularly imprinted polymers (MMIPs) were prepared for cocaine recognition by bulk polymerization in the presence of magnetic nanoparticles (MNPs). Two reagents (polyethylene glycol (PEG) and 3-(trimethoxysilyl)propyl methacrylate (V)) were used for MNPs modification. MMIPs were characterized and compared in terms of loading capacity, reusability, accuracy and precision for the extraction of cocaine from saliva samples. It was observed that V-MMIPs gave higher physical stability than PEG-MMIPs. Thus, V-MMIP were used for the analysis of cocaine users saliva. The developed procedure based on ion mobility spectrometry (EMS) provided limits of detection and quantification of 4 and 14 mu g L-1, respectively, and recoveries in cocaine free saliva samples spiked at 80, 270 and 560 mu g L-1 ranging from 80 to 99%. Results found by the proposed method were statistically comparable to those obtained by two reference procedures; a lateral flow immunoassay and an ultra-high performance liquid chromatography coupled with tandem mass spectrometry. Therefore, MMIP-IMS can be considered as a fast, selective and sensitive alternative to reference methods with affordable cost avoiding the requirement of skilled operator. (C) 2018 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据