期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 120, 期 2, 页码 1133-1140出版社
WILEY
DOI: 10.1002/jcb.26659
关键词
inflammation; microRNAs; NF-B; rheumatoid arthritis; TLR4
资金
- National Natural Science Foundation of China [81601408, 8167060388]
- Shandong Natural Science Foundation for Young Scholars [ZR2016HQ12]
- Shandong Medical and Health Science and Technology Development Program [2015WS0052, 2016WS0681, 2016WS0686, 2016WS0689]
- Weifang Science and Technology Development Program [2017YX019]
Currently published studies have implicated that microRNAs (miRNAs) including exosomes-encapsulated miRNAs play a critical role in rheumatoid arthritis (RA). Previously, we have found that exosomes-encapsulated miR-548a-3p was significantly decreased in serum samples from RA patients by miRNAs microarray analysis. However, little is known of the role of miR-548a-3p in the development and progression of RA. In this study, we aim to investigate the underlying molecular mechanisms of miR-548a-3p in RA, which will provide new insight into understanding the pathogenesis of RA and identifying novel therapeutics targets for this disease. As validated by quantitative real-time polymerase chain reaction (qRT-PCR), the expression of miR-548a-3p in serum exosomes and peripheral blood mononuclear cells (PBMCs) of RA patients (n=76) was obviously down-regulated compared with healthy controls (n=20). Serum exosomal miR-548a-3p was negatively associated with levels of CRP, RF, and ESR in serum of patients with RA. MiR-548a-3p could inhibit the proliferation and activation of pTHP-1 cells by regulating the TLR4/NF-B signaling pathway. Accordingly, exosomes-delivered miR-548a-3p may be a critical factor predicting the disease activity of RA. MiR-548a-3p/TLR4/NF-B axis can serve as promising targets for RA diagnosis and treatment.
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