期刊
JOURNAL OF CELL SCIENCE
卷 131, 期 6, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.214411
关键词
H2afx; Mdc1; Crossover; X-Y; Meiosis; Checkpoint
类别
资金
- Fondazione Telethon Grant [GGP12189]
- PRIN from Ministero Italiano dell'Universita e della Ricerca Scientifica [2010M4NEFY_004]
- Mission Sustainability grant from the Universita degli Studi di Roma Tor Vergata [141]
In somatic cells, H2afx and Mdc1 are close functional partners in DNA repair and damage response. However, it is not known whether they are also involved in the maintenance of genome integrity in meiosis. By analyzing chromosome dynamics in H2afx(-/-) spermatocytes, we found that the synapsis of autosomes and X-Y chromosomes was impaired in a fraction of cells. Such defects correlated with an abnormal recombination profile. Conversely, Mdc1 was dispensable for the synapsis of the autosomes and played only a minor role in X-Y synapsis, compared with the action of H2afx. This suggested that those genes have non-overlapping functions in chromosome synapsis. However, we observed that both genes play a similar role in the assembly of MLH3 onto chromosomes, a key step in crossover formation. Moreover, we show that H2afx and Mdc1 cooperate in promoting the activation of the recombination-dependent checkpoint, a mechanism that restrains the differentiation of cells with unrepaired DSBs. This occurs by a mechanism that involves P53. Overall, our data show that, in male germ cells, H2afx and Mdc1 promote the maintenance of genome integrity.
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