期刊
JOURNAL OF CELL BIOLOGY
卷 217, 期 8, 页码 2831-2849出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201711104
关键词
-
类别
资金
- National Institutes of Health [R01EY018421]
- Fight for Sight
- Research to Prevent Blindness
- Lions Clubs of Central New York, District 20-Y1
Physical properties of primary cilia membranes in living cells were examined using two independent, high-spatiotemporal-resolution approaches: fast tracking of single quantum dot-labeled G protein-coupled receptors and a novel two-photon super-resolution fluorescence recovery after photobleaching of protein ensemble. Both approaches demonstrated the cilium membrane to be partitioned into corralled domains spanning 274 +/- 20 nm, within which the receptors are transiently confined for 0.71 +/- 0.09 s. The mean membrane diffusion coefficient within the corrals, D-m1 = 2.9 +/- 0.41 mu m(2)/s, showed that the ciliary membranes were among the most fluid encountered. At longer times, the apparent membrane diffusion coefficient, D-m2 = 0.23 +/- 0.05 mu m(2)/s, showed that corral boundaries impeded receptor diffusion 13-fold. Mathematical simulations predict the probability of G protein-coupled receptors crossing corral boundaries to be 1 in 472. Remarkably, latrunculin A, cytochalasin D, and jasplakinolide treatments altered the corral permeability. Ciliary membranes are thus partitioned into highly fluid membrane nanodomains that are delimited by filamentous actin.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据