期刊
JOURNAL OF CARBOHYDRATE CHEMISTRY
卷 37, 期 4, 页码 199-209出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/07328303.2018.1451875
关键词
glycan-binding protein; concanavalin A; fluorescence resonance energy transfer; gold nanoparticles; quantum dots
资金
- National Natural Science Foundation of China [31770862, 81661148055, 31470801]
- National Key R & D Program of China [2016YFE0205800]
- Open Project Program of the State Key Laboratory of Microbial Resources, Institute of Microbiology, CAS [SKLMR-20160604]
Many pathogens can use their glycan-binding proteins (GBPs) to interact with glycan receptors on host cell surface. Here, a novel strategy is presented for the detection of GBPs based on fluorescence resonance energy transfer (FRET). Using mannosefunctionalized quantum dots and mannose-functionalized gold nanoparticles, a FRET assay system was developed and a mannose-specific GBP concanavalin A could be detected in 5 min with a detection limit of 3 nM. This result indicated the potential for rapid and sensitive biosensing of various kinds of GBPs and pathogens by changing the glycan structure on the surface of QDs and AuNPs. [GRAPHICS] .
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据