期刊
JOURNAL OF BONE AND MINERAL METABOLISM
卷 37, 期 2, 页码 212-223出版社
SPRINGER JAPAN KK
DOI: 10.1007/s00774-018-0919-y
关键词
Renal osteodystrophy; Vascular calcification; Bone; Osteocyte; Mineralisation defects
资金
- FRQ-S [32661]
- KRESCENT program from the CIHR-Kidney Foundation of Canada
- Kidney Foundation of Canada [KFOC160013]
- Department of Medicine from Universite Laval
- la Fondation du CHU de Quebec from Universite Laval
A link between vascular calcification and bone anomalies has been suggested in chronic kidney disease (CKD) patients with low bone turnover disease. We investigated the vascular expression of osteocyte markers in relation to bone microarchitecture and mineralization defects in a model of low bone turnover CKD rats with vascular calcification. CKD with vascular calcification was induced by 5/6 nephrectomy followed by high calcium and phosphate diet, and vitamin D supplementation (Ca/P/VitD). CKD+Ca/P/VitD group (n=12) was compared to CKD+normal diet (n=12), control+normal diet (n=8) and control+Ca/P/VitD supplementation (n=8). At week 6, tibia, femurs and the thoracic aorta were analysed by Micro-Ct, histomorphometry and for expression of osteocyte markers. High Ca/P/VitD treatment induced vascular calcification only in CKD rats, suppressed serum parathyroid hormone levels and led to higher sclerostin, DKK1 and FGF23 serum levels. Expression of sclerostin, DKK1 and DMP1 but not FGF23 were increased in calcified vessels from CKD+Ca/P/VitD rats. Despite low parathyroid hormone levels, tibia bone cortical thickness was significantly lower in CKD+Ca/P/VitD rats as compared to control rats fed a normal diet, which is likely the result of radial growth impairment. Finally, Ca/P/VitD treatment in CKD rats induced a bone mineralization defect, which is likely explained by the high calcitriol dose. In conclusion, Ca/P/VitD supplementation in CKD rats induces expression of osteocyte markers in vessels and bone mineralisation anomalies. Further studies should evaluate the mechanisms of high dose calcitriol-induced bone mineralisation defects in CKD.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据