期刊
JOURNAL OF BIOTECHNOLOGY
卷 271, 期 -, 页码 1-7出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2018.02.007
关键词
Chiral alcohol; Ketoreductase; Cofactor regeneration; Magnetic Fe3O4 nanoparticles; Magnetic Combi-CLEAs
资金
- China Postdoctoral Science Foundation [2016M600417, 2017T100373]
- 333 project of Jiangsu Province [BRA2017458]
- Six Talent Peaks Project in Jiangsu Province [2015-JY-016]
- Priority Academic Program Development of Jiangsu Higher Education Institutions, PAPD
Magnetic Fe3O4 nanoparticles were prepared and embedded into the Combi-CLEAs to produce the magnetic Combi-CLEAs in this work. The process for magnetic Combi-CLEAs preparation was optimized, and its properties were investigated. The optimum temperature, thermal stability and optimum pH of magnetic Combi-CLEAs were similar to those of Combi-CLEAs. The catalytic performance of magnetic Combi-CLEAs was tested with the biosynthesis of (S)-ethyl 4-chloro-3-hydroxybutyrate ((S)-CHBE). Magnetic Combi-CLEAs could tolerate higher substrate concentration in the biphasic system. The catalytic efficiency and long-term operational stability of magnetic Combi-CLEAs were obviously superior to those of Combi-CLEAs in both aqueous and biphasic systems. Embedding of magnetic Fe3O4 nanoparticles endowing rigidity contributed to these improvements. Furthermore, the preparation of magnetic Combi-CLEAs was easy, and its recovery during multiple batches of reactions could be fulfilled by magnetic field. Aforementioned advantages make the magnetic Combi-CLEAs hold obvious potential for industrial application.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据