期刊
JOURNAL OF BIOTECHNOLOGY
卷 278, 期 -, 页码 34-38出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2018.05.007
关键词
Non-proteinogenic amino acid; Phenylglycine; Aminotransferase; Antibiotics; Pristinamycin
资金
- University of Tubingen (Deutsche Forschungsgemeinschaft) [ZUK 63]
- Springer Best Masters Award
L-phenylglycine is a rare non-proteinogenic amino acid, which only occurs in a few natural compounds, such as the streptogramin antibiotics pristinamycin I and virginiamycin S or the bicyclic peptide antibiotic dityromycin. Here we report on the biochemical characterization of the aminotransferase PglE that catalyzes the transamination from phenylglyoxylate to L-phenylglycine, which represents the final reaction step during phenylglycine biosynthesis. Enzyme assays with the purified PglE enzyme revealed that L-phenylalanine is used as an amino group donor for the transamination reaction, leading to the formation of phenylpyruvate, which may re-enter phenylglycine biosynthesis as a precursor. Based on these results, we postulate a novel L-phenylglycine biosynthetic pathway.
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