期刊
JOURNAL OF BIOTECHNOLOGY
卷 267, 期 -, 页码 71-78出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2017.12.022
关键词
Ohmic heating; Algal biodiesel; Transesterification; Biodiesel; Chlorella sp.; Taguchi method
资金
- Kasetsart University Research and Development Institute, Kasetsart University, Thailand
- Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission
Suspensions of the model microalga Chlorella sp. TISTR 8990 were pretreated by ohmic heating to facilitate release of lipids from the cells in subsequent extraction and lipase-mediated transesterification to biodiesel. After ohmic pretreatment, the moist biomass was suspended in a system of water, hexane, methanol and immobilized lipase for extraction of lipids and simultaneous conversion to biodiesel. The ohmic pretreatment was optimized using an experimental design based on Taguchi method to provide treated biomass that maximized the biodiesel yield in subsequent extraction-transesterification operation. The experimental factors were the frequency of electric current (5-105 Hz), the processing temperature (50-70 degrees C), the algal biomass concentration in the slurry (algal fresh weight to water mass ratio of 1-3) and the incubation time (1-3 min). Extraction-transesterification of the pretreated biomass was carried out at 40 degrees C for 24 h using a reaction systems of a fixed composition (i.e. biomass, hexane, methanol, water and immobilized enzyme). Compared to control (i.e. untreated biomass), the ohmic pretreatment under optimal conditions (5 Hz current frequency, 70 degrees C, 1: 2 mass ratio of biomass to water, incubation time of 2-min) increased the rate of subsequent transesterification by nearly 2-fold.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据