期刊
JOURNAL OF BIOTECHNOLOGY
卷 275, 期 -, 页码 24-30出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2018.04.001
关键词
Alcohol stress; Aureobasidium pullulans; Glyoxylate shunt; Malate synthase; Polymalic acid
资金
- National Natural Science Foundation of China [31571816]
- National High Technology Research and Development Program of China (863 Program) [2015AA021005]
- Chongqing Social and People's Livelihood Guarantee Special Program [cstc2016shmszx80075]
- State Key Laboratory of Materials-Oriented Chemical Engineering (Nanjing Tech University), China [KL15-10]
Polymalic acid (PMA) is a water-soluble biopolymer produced by the yeast-like fungus Aureobasidium pullulans. In this study, the physiological response of A. pullulans against exogenous alcohols stress was investigated. Interestingly, ethanol stress was an effective inducer of enhanced PMA yield, although cell growth was slightly inhibited. The stress-responsive gene malate synthase (mls), which is involved in the glyoxylate shunt, was identified and was found to be regulated by exogenous ethanol stress. Therefore, an engineered strain, YJ-MLS, was constructed by overexpressing the endogenous mls gene, which increased the PMA titer by 16.2% compared with the wild-type strain. Following addition of 1% (v/v) of ethanol, a high PMA titer of 40.0 +/- 0.38 g/L was obtained using batch fermentation with the mutant YJ-MLS in a 5-L fermentor, with a strongest PMA productivity of 0.56 g/L h. This study was the interesting report to show strengthening of the carbon metabolic flow from the glyoxylate shunt for PMA synthesis, and also provided a new sight for re-recognizing the regulatory behavior of alcohol stress in eukaryotic microbes.
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