期刊
JOURNAL OF BIOTECHNOLOGY
卷 274, 期 -, 页码 47-53出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2018.03.006
关键词
Phycoerythrobilin; Escherichia coli; Biliverdin; Heme oxygenase; Ferredoxin-dependent bilin reductase; Foam separation
资金
- German-Israeli-Foundation for Scientific Research and Development
- Deutsche Forschungsgemeinschaft [UL 170/16-1]
Phycoerythrobilin (PEB) is an open-chain tetrapyrrole derived from heme and plays an important role as light-harvesting pigment in the phycobiliproteins of cyanobacteria and red algae. Furthermore, PEB can also function as an antioxidant with potential use as a natural acid stable food colorant. PEB is not commercially available and large, pure quantities can only be obtained by laborious methanolysis of red algae followed by liquid chromatography. Here we describe an improved method for high yield production and purification of PEB in Escherichia coli via heterologous expression where the two required enzymes heme oxygenase and PEB synthase subsequently convert the substrate heme provided by the host cell. Experiments in shaking flasks resulted in the highest product yield of 680.23 +/- 42.75 mu g PEB per g cell dry weight, by induction with 0.1mM IPTG. Scale-up to batch-operated fermentation in a 2 L bioreactor reached product concentrations up to 5.02 mg PEB L-1 by adjustment of aeration, induction time, media composition and supplementation of precursors. A further approach included separation of PEB from developed foam above the culture. This enabled continuous product collection during cultivation and simplified product purification. Produced PEB was validated via UV-vis spectroscopy, high pressure liquid chromatography and mass spectrometry.
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