期刊
JOURNAL OF BIOSCIENCES
卷 43, 期 3, 页码 555-567出版社
INDIAN ACAD SCIENCES
DOI: 10.1007/s12038-018-9763-4
关键词
Fluorescence; fluorescence anisotropy; fluorescence lifetime; fluorescence quenching; Maximum Entropy Method; sample heterogeneity; time-resolved fluorescence
类别
This article describes the basic principles of steady-state and time-resolved fluorescence. The formal equivalence of the two methodologies is described first, followed by the extra advantages of time-resolved methods in revealing population heterogeneity in complex systems encountered in biology. Several examples from the author's work are described in support of the above contention. Finally, several misinterpretations and pitfalls in the interpretation of fluorescence data and their remedy are described.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据